Resolution of small G1 and G2 populations of L1210 leukemia by flow microfluorometric analysis aided by velocity sedimentation

article

Edelstein, M.

Lelieveld, P.
L1210 leukemic cells grown in vitro were subjected to kinetic analysis using a flow microfluorometer. A single broad peak was found for the DNA content distribution if unfractionated cells were used; prior fractionation using 1 g velocity sedimentation allowed the separation of small peaks with smaller (G1) and larger (G2) DNA contents from the dominant S phase peak with intermediate DNA content.
Chemicals/CAS: DNA, 9007-49-2; DNA, Neoplasm
Topics





















DNAcancer cell culturecell cyclecell kineticsin vitro studyleukemia l 1210methodologymousetheoretical studyanimaldensity gradient centrifugationfluorometrykineticsleukemia L 1210AnimalCentrifugation, Density GradientDNA, NeoplasmFluorometryIn VitroKineticsLeukemia L1210Support, U.S. Gov't, P.H.S.
TNO Identifier
228251
Repository link
https://resolver.tno.nl/uuid:7e638e72-ece3-4997-b608-61f53c7ed877
ISSN
00144754
Source
Experientia, 33(12), pp. 1604-1605.
Pages
1604-1605
Files
To receive the publication files, please send an e-mail request to TNO Repository.