The aneurinpyrosphosphate content of red and white blood corpuscles in the rat and in man, in various states of aneurin provision and in disease
article
1. Methods are described for accurate counting of red and white blood cells for the determination of the aneurinpyrophosphate (APP) content of these cells. 2. These methods were applied to: a) rat blood (adequately nourished rats and rats on an aneurin-deficient diet); b) human blood (healthy subjects on their usual diet or on an aneurin-deficient diet, and patients). Moreover APP was determined in liver, kidney, brain abd leg-muscle of the rats.
3. The mean values of the APP contents of the blood cells and the tissues of the adequately nourished rats were: red cells: 2.1 γ per 1011 cells; white cells: 340 γ per 1011 cells; liver: 12.5 γ per g; kidney: 7.0 γ per g; brain: 3.8 γ per g; leg-muscle: 2.4 γ per g. For rats after 5 days on an aneurin-free diet these values were: red cells: 1.0 γ per 1011 cell; white cells: 240 γ per 1011 cells; liver: 4.1 γ per g; kidney: 2.7 γ per g; brain: 3.1 γ per g; leg-muscle: 1.25 γ per g. In the well-fed rat an average leucocyte contains 160 times as much APP as an average erythrocyte. After 5 days without aneurin the ration of the contents of a white and a red cell has risen to 240. So on an aneurin-free diet the content on an aneurin-free diet the content of the red cells decreases more rapidly than the content of the white cells.
3. The mean values of the APP contents of the blood cells and the tissues of the adequately nourished rats were: red cells: 2.1 γ per 1011 cells; white cells: 340 γ per 1011 cells; liver: 12.5 γ per g; kidney: 7.0 γ per g; brain: 3.8 γ per g; leg-muscle: 2.4 γ per g. For rats after 5 days on an aneurin-free diet these values were: red cells: 1.0 γ per 1011 cell; white cells: 240 γ per 1011 cells; liver: 4.1 γ per g; kidney: 2.7 γ per g; brain: 3.1 γ per g; leg-muscle: 1.25 γ per g. In the well-fed rat an average leucocyte contains 160 times as much APP as an average erythrocyte. After 5 days without aneurin the ration of the contents of a white and a red cell has risen to 240. So on an aneurin-free diet the content on an aneurin-free diet the content of the red cells decreases more rapidly than the content of the white cells.
TNO Identifier
353479
Source
Biochimica et Biophysica Acta, 3, pp. 44-64.
Pages
44-64
Files
To receive the publication files, please send an e-mail request to TNO Repository.