The temperature characteristics of spore germination and growth of bacillus cereus
article
Spore suspensions of Bacillus cereus, strain Smith 232, free from vegetative cells and spore clumps, were prepared by repeated washing at pH 3·0 alternated with ultrasonic radiation treatment. The germination of spores suspended in yeast extract phosphate broth of pH 7·O and incubated at different temperatures was followed by phase-contrast microscopy.
Spores gave 85 % germination after 6 hr at 8° in this medium, though no growth of young vegetative cell inocula of the same strain occurred in yeast extract glucose phosphate broth after 4 months at the same temperature. It is suggested that the lack of increase in numbers of spore formers, present in foods stored under refrigeration, is due to inhibition of vegetative growth rather than to prevention of spore germination.
Spores gave 85 % germination after 6 hr at 8° in this medium, though no growth of young vegetative cell inocula of the same strain occurred in yeast extract glucose phosphate broth after 4 months at the same temperature. It is suggested that the lack of increase in numbers of spore formers, present in foods stored under refrigeration, is due to inhibition of vegetative growth rather than to prevention of spore germination.
TNO Identifier
49722
Source
The Journal of Applied Bacteriology, 20(3), pp. 454-459.
Pages
454-459
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