A double fluorescence method for determining doxorubicin distribution and vascular supply in the mouse kidney
article
Freeze-sectioning followed by freeze-drying completely preserves the topographic distribution of soluble substances, with the additional advantage of permitting permanent mounting. This rapid and effective technique was applied to study the distribution of fluorescence of the cytotoxic drug Doxorubicin in microscopic slices from in vivo treated animals. A fluorescent conjugate of different fluorescence, Blankophor-G-Dextran, which remained in the vessels, was used in the same living animals to characterize the vascular supply in the kidney of treated mice in relation to Doxorubicin distribtion.
Chemicals/CAS: doxorubicin, 23214-92-8, 25316-40-9; Blankophor G, 7426-67-7; Doxorubicin, 23214-92-8; Fluorescent Dyes; Triazoles
Chemicals/CAS: doxorubicin, 23214-92-8, 25316-40-9; Blankophor G, 7426-67-7; Doxorubicin, 23214-92-8; Fluorescent Dyes; Triazoles
Topics
doxorubicinanimal experimentcardiovascular systemcytologydrug distributionfluorescence microscopykidneymousenonhumanpharmacokineticspriority journalvascularizationAnimalCell NucleusCytoplasmDoxorubicinFluorescent DyesFreeze DryingFrozen SectionsHistocytochemistryKidneyKidney GlomerulusKineticsMaleMiceMice, Inbred C57BLMicroscopy, FluorescenceStaining and LabelingSupport, Non-U.S. Gov'tTriazoles
TNO Identifier
229826
ISSN
00221554
Source
Journal of Histochemistry and Cytochemistry, 33(1), pp. 73-76.
Pages
73-76
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