Major histocompatibility complex class II DQ diversity in Rhesus macaques
article
By the use of restriction fragment length polymorphism analysis 10 Taq 1 fragments could be identified for the MhcMamu-DQA1 region. A strong correlation exists between the occurrence of Mamu-DQA1/Taq I fragments and Mamu-DAQ1 allelic sequence variation. Most restriction fragments correspond with a unique Mamu-DQA1 allele, with one exception being the Taq I 4.5 kb fragment that is associated with two Mamu-DQA1 alleles. The RFLP technique allowed the identification of 15 Mamu-DQB1/Taq I restriction fragments, whereas sequence analysis has permitted the characterization of at least 20 different Mamu-DQB1 alleles. In this communication two unpublished Mamu-DQB1 sequences are described. For Mamu-DQB1, on only four occasions was it possible to demonstrate a correlation between a certain fragment and an allelic sequence. These analyses, performed on material from truly homozygous animals, allowed us to define which combinations of Mamu-DQA1 and DQB1 molecules form heterodimers at the cell surface. In addition, these studies are helpful in typing non-human primate species that are used in biomedical research. Chemicals/CAS: DNA, 9007-49-2; HLA-DQ Antigens; HLA-DQA1; HLA-DQB1
Topics
MhcMhcMamuRhesus macaqueRPLPHLA DQ antigenmajor histocompatibility antigen class 2allelearticlegene sequencegenetic analysisgenetic variabilitymajor histocompatibility complexnonhumanpriority journalrestriction fragmentrestriction fragment length polymorphismrhesus monkeyAllelesAmino Acid SequenceAnimalBase SequenceBlotting, SouthernDNAHLA-DQ AntigensHomozygoteMacaca mulattaMolecular Sequence DataPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthSupport, Non-U.S. Gov't
TNO Identifier
232089
ISSN
0001-2815
Source
Tissue Antigens, 41(4), pp. 178-185.
Pages
178-185
Files
To receive the publication files, please send an e-mail request to TNO Repository.