Semi-automated fluorometric determination of pyridoxal-5'-phosphate (vitamin B6) in whole blood by high-performance liquid chromatography (HPLC)

article


Schrijver, J.

Speek, A.J.

Schreurs, W.H.P.
A sensitive and reliable method for the determination of vitamin B6 as pyridoxal-5'-phosphate (PLP) in whole blood is described. After acid extraction of PLP, a fully automated high-performance liquid chromatographic (HPLC) system is used to separate PLP from interfering compounds. PLP is detected fluorometrically as its semicarbazone. By calculating the concentration of PLP on-line with the aid of a computer, it is possible to run one hundred and twenty-five samples within forty-eight hours in routine analysis. The within-assay and the between-assay coefficients of variation of the analysis of PLP in whole blood were 3.3 and 4.7% respectively. The between-assay analytical recovery of PLP added to whole blood was 100.0 +/- 7.1% (mean +/- SD). Reference values of PLP in human blood were found in the range 55-110 nmol/l. Chemicals/CAS: pyridoxal 5 phosphate, 54-47-7; Pyridoxal Phosphate, 54-47-7; Pyridoxal, 66-72-8
Topics


















Pyridoxal 5 phosphateBloodBlood and hemopoietic systemDrug blood levelDrug determinationFluorometryHigh performance liquid chromatographyIn vitro studyMethodologyAdultChromatography, High Pressure LiquidComputersFemaleHumanMalePyridoxalPyridoxal PhosphateReference ValuesSpectrometry, Fluorescence
TNO Identifier
229082
Repository link
https://resolver.tno.nl/uuid:28feef60-0d51-401a-b369-092f048e5781
ISSN
03009831
Source
International Journal for Vitamin and Nutrition Research, 51(3), pp. 216-222.
Pages
216-222
Files
To receive the publication files, please send an e-mail request to TNO Repository.