Effect of various thymic and nonthymic factors on in vitro antibody formation by spleen cells from nude mice

article



Blankwater, M.J.

Levert, L.A.

Swart, A.C.W.

Bekkum, D.W. van
Thymosin and thymic humoral factor (THF), both prepared from whole thymus tissue, increased the in vitro anti-SRBC response of spleen cells from nu/nu mice. Lymphoid and nonlymphoid control preparations did not influence the response. Standard isolation procedures for thymosin and THF were employed to prepare factors from thymus lymphocytes (TL) separated from calf thymus and from thymus tissue enriched in thymus epithelium (TE) by preirradiation of the calves. The activity of the TE preparations was greater than that of the total thymus (TT) preparations. The TL preparations were marginally effective. However, the activity of the TE preparations could not be attributed exclusively to epithelium-derived factors, since spleen and lymph node extracts from the irradiated calves contained stimulatory material of an unknown origin which may also be present in the TE preparations.
Topics





















thymosinthymus factorantibody productionerythrocytein vitro studylymph nodemousespleenspleen cellthymusAnimalAntibody FormationCells, CulturedFemaleMaleMiceMice, Inbred C57BLMice, Inbred CBAMice, NudeSpleenThymosinThymus Hormones
TNO Identifier
228337
Repository link
https://resolver.tno.nl/uuid:4ccdb11f-e684-4b30-8f37-c5210940f9af
ISSN
00088749
Source
Cellular Immunology, 35(2), pp. 242-252.
Pages
242-252
Files
To receive the publication files, please send an e-mail request to TNO Repository.