Different inherited levels of DNA repair replication in xeroderma pigmentosum cell strains after exposure to ultraviolet irradiation
article
Primary fibroblast cultures were established from 8 patients having different degrees of clinical symptoms of xeroderma pigmentosum. Repair replication after exposure of cells to different doses of ultraviolet irradiation (predominantly 254 nm) was studied by means of [3H] thymidine labeling and autoradiography. A decreased repair DNA synthesis in cells in G1 and G2 phase was found in all xeroderma pigmentosum cell cultures relative to control cell cultures obtained from healthy people. Cell strains originating from two severe cases of xeroderma showed no repair synthesis after short autoradiographic exposure times, although, after one-month exposure a slight labeling was observed (10-20% of the control). The repair activity in cells from the other patients ranged from 70% for a light case to 50 and 30% for moderate cases of the disease. Cells obtained from related patients showed identical levels of repair activity, suggesting a genetically determined constant level of reduced repair replication. A xeroderma cell strain transformed by SV40 virus showed no repair replication as did the original strain before transformation. © 1970.
Topics
TritiumAdolescentAdultAutoradiographyChildCulture mediumDNA replicationEnzymologyFemaleFibroblastGeneticsHumanIn vitro studyMaleMetabolismMolecular biologyMutationRadiation exposureRadiation responseStainingUltraviolet radiationXeroderma pigmentosumChildCulture MediaDNA ReplicationFemaleFibroblastsHumanIn VitroMaleMiddle AgeMolecular BiologyMutationRadiation EffectsStaining and LabelingTritiumUltraviolet RaysXeroderma Pigmentosum
TNO Identifier
227171
ISSN
00275107
Source
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 9(5), pp. 507-516.
Pages
507-516
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