Selective and sensitive trace analysis of sulfur mustard with thermal desorption and two-dimensional gas chromatography-mass spectrometry
article
An improved method is presented for the trace analysis of sulfur mustard (HD) in biological samples, such as blood and tissue from laboratory animals. Using the internal standard method and liquid-liquid extraction with ethyl acetate, up to 400 μL of the extract was injected by thermal desorption from Tenax and analyzed by two-dimensional GC-MS/EI in SIM mode. The analysis was compared with a direct GC injection. Reversed thermal desorption was used as a tool for handling heavily contaminated (fat) samples, thus preventing contamination of the injection system and pre-column. A successful analytical configuration has been set up for the bioanalysis of HD at the low, toxicologically relevant pM level. A detection limit of 10 pg mL-1 blood or pg g-1 tissue of sulfur mustard (S/N = 3) was established by using this configuration. © 2006 Elsevier B.V. All rights reserved.
Topics
Acetic acid ethyl ester, 141-78-6Mustard gas, 505-60-2Biological samplesMass spectrometrySample preparationSulfur mustardThermal desorptionToxicokineticsTwo-dimensional GCBiomedical engineeringExtractionGas chromatographyMass spectrometryThermal desorptionToxicityBiological samplesSample preparationSulfur mustard (HD)ToxicokineticsSulfur compoundsAnimal experimentAnimal tissueBlood samplingChemical analysisContaminated animalControlled studyDesorptionExperimental animalGas chromatographyLiquid liquid extractionMass spectrometryNonhumanThermal analysisToxicologyAnimalsGas chromatographyHaplorhiniMass spectrometryReproducibility of resultsAnimalia
TNO Identifier
183404
ISSN
0021-9673
Source
Journal of Chromatography A, 1150(1-2), pp. 62-69.
Pages
62-69
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