Rapid and simple methods for the investigation of lipoxygenase pathways in human granulocytes
article
Metabolites of arachidonic acid (or analogues), produced by the lipoxygenase pathway, play an important role in many physiological and pathophysiological processes. However, many questions related to their metabolism and exact function remain unanswered. Current methods for the measurement of arachidonic acid metabolites require large blood samples and time-consuming extraction and concentration procedures. Moreover, high-performance liquid chromatographic (HPLC) methods applied to the separation of lipoxygenase products use complex gradients and give long retention times. In view of future research in this area, an urgent need exists for rapid and convenient micromethods. This paper describes a rapid HPLC method for the determination of leukotrienes and hydroxy fatty acids originating from in vitro stimulated peripheral blood cells. The advantages of the system are that (i) the use of microtitre plates enables a large number of experiments to be performed very efficiently, (ii) no time-consuming extraction or concentration procedures are required before HPLC analysis and (iii) the sample capacity is 144 per day.
Chemicals/CAS: lipoxygenase, 9027-17-2, 9029-60-1; Fatty Acids; Leukotriene B4, 71160-24-2; Leukotriene E4, 75715-89-8; Lipoxygenase, EC 1.13.11.12; SRS-A
Chemicals/CAS: lipoxygenase, 9027-17-2, 9029-60-1; Fatty Acids; Leukotriene B4, 71160-24-2; Leukotriene E4, 75715-89-8; Lipoxygenase, EC 1.13.11.12; SRS-A
Topics
TNO Identifier
230564
ISSN
03784347
Source
Journal of Chromatography - Biomedical Applications, 431(2), pp. 413-417.
Pages
413-417
Files
To receive the publication files, please send an e-mail request to TNO Repository.