Mobilization of leukemic cells from tissue stores by synthetic polyanions: Experimental and clinical data
article
Studies on synthetic polyanion induced mobilization of leukemic cells from the tissue stores to the blood were initiated in attempts to increase the efficacy of the treatment of leukemia by extracorporeal irradiation of the blood. Within 1-5 h after i.v. injection of dextran sulphate, it was found that, in both a rat model for acute myelocytic leukemia and in human chronic lymphocytic leukemia, a two to three fold increase in the number of leukemic cells, which is reversible within the next 3-5 h, occurs in the circulating blood. Besides leukemic cells normal lymphocytes are also mobilized. Toxicological studies in normal rhesus monkeys indicate that the major side effect is a heparin like anticoagulant activity within clinically acceptable limits. The mechanism of cell mobilization is explained at present in terms of an altered electronegative charge of the cell surface on binding to polyanions which may change the interaction with surrounding structures and lead to an increased release to the blood. This hypothesis is supported by the observations that [1] the electrophoretic mobility of cells increases after in vitro incubation with polyanions and [2] the in vivo cell mobilization phenomenon can be promptly abolished by administering polycations.
Topics
TNO Identifier
228039
ISSN
03404684
Source
Blood Cells, 2(3), pp. 437-451.
Pages
437-451
Files
To receive the publication files, please send an e-mail request to TNO Repository.