An ear swelling reaction for in vivo monitoring of inflammatory T cell activity in collagen-arthritic rats
article
T cells play a dominant role in the pathogenesis of collagen-induced arthritis (CIA). The effector mechanism involves a delayed-type hypersensitivity reaction in the synovial joint. Here we describe a new test system, named a flare reaction, to monitor directly this inflammatory T cell function. It is shown that type II collagen (CII) injected into the pinna of the ear is a target for CII-reactive inflammatory T cells formed during the early pathogenesis of CIA. The inflammatory swelling of the joint and the CII-injected ear in CIA rats develop in a coordinated manner. This assay greatly facilitates monitoring of in vivo activated inflammatory T cells during the induction of CIA.
Topics
Collagen-induced arthritisT cellCollagen type 2Animal experimentAnimal modelArthritisEarImmunizationInflammatory cellNonhumanPriority journalRatT lymphocyteAnimalArthritis, ExperimentalCollagenEar, ExternalEdemaHypersensitivity, DelayedImmunizationImmunosuppressionOvalbuminRatsRats, Inbred StrainsT-LymphocytesAnimaliaPinnaCollagen, 9007-34-5Ovalbumin, 9006-59-1
TNO Identifier
232809
ISSN
00221759
Source
Journal of Immunological Methods, 176(2), pp. 179-184.
Pages
179-184
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