Genetic study of prophage excision with a temperature inducible mutant of Mu-1

article

Schröder, W.

Putte, P. van de
Escherichia coli strains, carrying F'episomes with a thermoinducible prophage of Mu were used to study the effect of heat induction on the ability of the cells to transfer these episomes to recipient cells. With this method information was obtained on the excision of the prophage after induction. Control studies were done with an episome containing a thermoinducible lambda prophage. The heat induction has opposite effects on the transfer of episomes containing Mu or λ: in the case of λ the number of sexductants in a λ-immune recipient is not significantly affected while sexduction into a non-immune recipient is increased by a factor 10-20. In contrast, the transfer of Mu-containing episomes into Mu-immune and also non-immune recipients is decreased by a factor of 100-200. The presented data exclude a precise "λ-like" excision of prophage Mu upon induction. A possible model for Mu excision is discussed. © 1974 Springer-Verlag.
Chemicals/CAS: DNA, Viral
Topics



















Virus DNABacterial chromosomeColiphageDNA virusEscherichia coliExtrachromosomal inheritanceGeneHeatMutationSexChromosomes, BacterialColiphagesDNA VirusesDNA, ViralEscherichia coliExtrachromosomal InheritanceGenesHeatMutationSex
TNO Identifier
227627
Repository link
https://resolver.tno.nl/uuid:d2b5e4a0-97e1-4944-98b8-a641f2a97eca
ISSN
00268925
Source
MGG Molecular & General Genetics, 130(2), pp. 99-104.
Publisher
Springer-Verlag
Pages
99-104
Files
To receive the publication files, please send an e-mail request to TNO Repository.