Rapid detection of hypoxanthine-guanine phosphoribosyltransferase on cellogel
article
A simple, fast and direct staining method for the detection of hypoxanthineguanine phosphoribosyltransferase is described. It is based on the conversion of inosine monophosphate to hypoxanthine, which is then enzymatically oxidized. This oxidation is coupled to the reduction of a tetrazolium salt to blue formazan. The electrophoretic pattern of HGPRT in man-Chinese hamster hybrid cells shows a three-banded pattern, suggesting a dimeric structure for this enzyme. © 1974 Springer-Verlag.
Chemicals/CAS: glycosyltransferase, 9033-07-2; guanine, 69257-39-2, 73-40-5; Guanine, 73-40-5; Hypoxanthines; Inosine Nucleotides; Pentosyltransferases, EC 2.4.2.
Chemicals/CAS: glycosyltransferase, 9033-07-2; guanine, 69257-39-2, 73-40-5; Guanine, 73-40-5; Hypoxanthines; Inosine Nucleotides; Pentosyltransferases, EC 2.4.2.
Topics
GlycosyltransferaseGuanineHypoxanthine derivativePurine nucleotideAnimalElectrophoresisHamsterHumanHybrid cellIn vitro studyOxidation reduction reactionStainingAnimalElectrophoresisGuanineHamstersHumanHybrid CellsHypoxanthinesIn VitroInosine NucleotidesOxidation-ReductionPentosyltransferasesStaining and Labeling
TNO Identifier
227619
ISSN
03406717
Source
Humangenetik, 22(1), pp. 75-80.
Publisher
Springer-Verlag
Pages
75-80
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