Properties of an ATP dependent deoxyribonuclease from Micrococcus luteus. A characterisation of the reaction products
article
In the early stage of degradation of T7 DNA by the ATP dependent DNAase from Micrococcus luteus the following products are formed: 1) Acid soluble, dialysable material representing approximately 35% of the degradation products. 2) Material of relatively low molecular weight and of heterogeneous size (from a few to about 300 nucleotides long) which is mainly acid insoluble and represents 15-20% of the degradation products. These molecules are for a large part single stranded, but a fraction, or possibly all of them, contains a double stranded region which may be as long as 150-300 nucleotides. 3) Material of relatively high molecular weight consisting of molecules with single stranded regions (on the average 80 nucleotides long) at their ends.
Chemicals/CAS: adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; deoxyribonuclease, 37211-67-9; Adenosine Triphosphate, 56-65-5; Deoxyribonucleases, EC 3.1.-; DNA, 9007-49-2; DNA, Single-Stranded; DNA, Viral; Exonucleases, EC 3.1.-; Phosphorus Radioisotopes
Chemicals/CAS: adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; deoxyribonuclease, 37211-67-9; Adenosine Triphosphate, 56-65-5; Deoxyribonucleases, EC 3.1.-; DNA, 9007-49-2; DNA, Single-Stranded; DNA, Viral; Exonucleases, EC 3.1.-; Phosphorus Radioisotopes
Topics
Adenosine triphosphateDeoxyribonucleaseIn vitro studyMicrococcus luteusMicroorganismTheoretical studyAdenosine TriphosphateAnimalBase SequenceCattleChromatography, AffinityChromatography, DEAE-CelluloseChromatography, GelColiphagesDeoxyribonucleasesDNADNA, Single-StrandedDNA, ViralEscherichia coliExonucleasesMicrococcusMolecular WeightNucleic Acid DenaturationNucleic Acid RenaturationPhosphorus RadioisotopesThymus Gland
TNO Identifier
227614
ISSN
00063002
Source
Biochimica et Biophysica Acta, 340(2), pp. 166-176.
Pages
166-176
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