The purification of nonparenchymal liver cell classes by centrifugal elutriation
bookPart
Nonparenchymal cell suspensions isolated from the rat liver were submitted to countercurrent centrifugation in an elutriator rotor. The distribution of endothelial cells, Kupffer cells, pit cells, fat-storing cells and leucocytes over the fractions obtained by centrifugal elutriation was determined. Two groups of Kupffer cells were collected at counterflow rates of 25.4 ml/min (fraction A) and 37.5 ml/m in (fraction B). This separation was made possible by slight differences in cell size and density. Expressed per 10^6 cells, the activities of the lysosomal enzymes acid phosphatase, S-acetylglucosaminidase, S-glucuronidase, cathepsin D and arginine naphthylamidase were 1.7 - 2.0 times higher in cells of fraction B than they were in cells of fraction A. The results indicate morphological and functional differences in the Kupffer cell population isolated from the rat liver.
TNO Identifier
356177
ISBN
0-4444-80031-X
Publisher
Elsevier
Source title
Kupffer cells and other liver sinusoidal cells
Editor(s)
Wisse, E.
Knook, D.L.
Knook, D.L.
Place of publication
Amsterdam
Pages
289-298
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