Isolated parenchymal, Kupffer and endothelial rat liver cells characterized by their lysosomal enzyme content
article
The activities of nine key lysosomal enzymes were determined in isolated parenchymal cells and in isolated Kupffer and endothelial cells purified by centrifugal elutriation. Compared with parenchymal cells, all specific lysosomal enzyme activities were much higher in Kupffer and endothelial cells. Kupffer cells showed a specific enrichment in acid lipase, β-glucuronidase, cathepsin D and aminopeptidase B, whereas the specific activities of acid phosphatase, acid DNAse, β-acetylglucosaminidase and arylsulphatase B were much higher in endothelial cells as compared with Kupffer cells; β-galactosidase was equally present in both cell classes. The distribution of lysosomal enzyme activities reflects the role of Kupffer and endothelial cells in the clearance and degradation of serum lipoproteins and glycoproteins. © 1980 Elsevier
TNO Identifier
353449
Source
Biochemical and biophysical research communications, 96(1), pp. 250-257.
Pages
250-257
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