Repair replication in human cells studied by sodium iodide isopycnic centrifugation of DNA in a fixed angle rotor
article
A sensitive and quantitative method is described for direct measurement of repair replication in UV irradiated human cells in tissue culture. The method is based on isopycnic centrifugation of repaired DNA in sodium iodide gradients in fixed angle rotors and determination of the amount of DNA by the fluorescence of a DNA ethidium bromide complex.
Chemicals/CAS: DNA, 9007-49-2; sodium iodide, 7681-82-5; Bromodeoxyuridine, 59-14-3; Cesium, 7440-46-2; DNA, 9007-49-2; Ethidium, 3546-21-2; Floxuridine, 50-91-9; Iodides; Tritium, 10028-17-8
Chemicals/CAS: DNA, 9007-49-2; sodium iodide, 7681-82-5; Bromodeoxyuridine, 59-14-3; Cesium, 7440-46-2; DNA, 9007-49-2; Ethidium, 3546-21-2; Floxuridine, 50-91-9; Iodides; Tritium, 10028-17-8
Topics
DNASodium iodideBroxuridine h 3Normal humanRadiotherapyTheoretical studyUltraviolet radiationBromodeoxyuridineCells, CulturedCentrifugation, Density GradientCesiumComparative StudyDNADNA RepairDNA ReplicationDose-Response Relationship, RadiationEthidiumEvaluation StudiesFloxuridineHumanIodidesRadiation EffectsSpectrometry, FluorescenceTime FactorsTritiumUltraviolet Rays
TNO Identifier
227513
ISSN
0003-2697
Source
Analytical Biochemistry, 54(1), pp. 178-187.
Pages
178-187
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