Pyrolysis-direct chemical ionization mass spectrometry of the dimorphic fungus Candida albicans and the pleomorphic fungus Ophiostoma ulmi

article



Tas, A.C.

Bastiaanse, H.B.

Greef, J. van der

Kerkenaar, A.
Pyrolysis-direct chemical ionization mass spectrometry was applied to investigate dimorphism-related differences in the fungi Candida albicans and Ophiostoma ulmi. In O. ulmi cell walls the yeast form contained more deoxyhexose (probably rhamnose) residues. This is in contrast to the results described for the related fungus Sporothrix schenckii. In C. albicans differences observed in the mass pyrograms of the yeast and hyphal forms decrease after application of the sterol biosynthesis inhibitor lombazole. This is in accordance with the observation that both forms tend to converge to a more common morphology. Besides the effect of the inhibitor on the cell biopolymer composition the primary effect on sterol biosynthesis could also be detected from the mass pyrograms. The presence of higher methylated sterols together with lower levels of ergosterol in spectra of the treated forms reflects the inhibition of the C-14 demethylation process by lombazole. © 1989.
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Candida albicansDirect chemical ionizationDiscriminant analysisFungiMass spectrometryOphiostoma ulmiPyrolysis.BiopolymersBiosynthesisMass Spectrometers--ApplicationsSugarsYeast--PyrolysisCandida albicansDeoxyhexoseDirect Chemical Ionization Mass SpectrometryLombazoleOphiostoma UlmiSterolsFungi
TNO Identifier
230896
Repository link
https://resolver.tno.nl/uuid:5b4d6331-a00e-4b0c-b1ce-e40f531b5ea0
ISSN
01652370
Source
Journal of Analytical and Applied Pyrolysis, 14(4), pp. 309-321.
Pages
309-321
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