Age-related induction and disappearance of carcinogen-DNA-adducts in livers of rats exposed to low levels of 2-acetylaminofluorene
article
It was investigated whether in vivo aging of rat liver is associated with changes in the induction and rate of disappearance of DNA damage. For this purpose 6- and 36-month-old rats were intraperitoneally injected with a single, low dose (5 mg/kg body wt) of the model liver carcinogen 2-acetylaminofluorene (AAF). Using the 32P-postlabeling assay we found that N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-C8-AF) was the major DNA-adduct formed. The minor adduct N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-C8-AAF) could only be detected after doses of 20 mg/kg or more. Quantitation of adduct levels at various time points after treatment indicated a rapid induction of AF-adducts, which were already present at 6 h after treatment. The subsequent loss of AF-adducts was relatively slow, as was indicated by the presence of a substantial amount of AF-adducts as late as 21 days after treatment. Slight age-related differences in the pattern of induction and disappearance of AF-adducts and a somewhat higher level of persisting lesions in old than in young rats were observed.
Chemicals/CAS: n (2 fluorenyl)acetamide, 27215-65-2, 53-96-3; 2-Acetylaminofluorene, 53-96-3; DNA, 9007-49-2; Phosphorus Radioisotopes
Chemicals/CAS: n (2 fluorenyl)acetamide, 27215-65-2, 53-96-3; 2-Acetylaminofluorene, 53-96-3; DNA, 9007-49-2; Phosphorus Radioisotopes
Topics
TNO Identifier
230835
ISSN
00092797
Source
Chemico-Biological Interactions, 69(4), pp. 373-384.
Pages
373-384
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