Determination of preservatives in cosmetic products. II. High-performance liquid chromatographic identification
article
A high-performance liquid chromatographic (HPLC) procedure is presented for the separation and identification of preservatives that are listed in the current EEC Council Directive on cosmetic products or have been permitted in the past. The method consists of an extraction of acidified cosmetics with methanol, and separation of the extracts by HPLC. Using two isocratic and two gradient reserved-phase HPLC systems, 47 preservatives were characterized by their retention times. The preservatives in three commercial cosmetic products were tentatively identified by the procedure described. The HPLC procedure is suitable for confirmation of the presence of preservatives in cosmetic products as indicated by a previously reported thin-layer chromatographic procedure. In general this method will permit the routine detection of preservatives in cosmetics in an approximate concentration of 0.01% (w/w).
Chemicals/CAS: chlorocresol, 1321-10-4, 59-50-7; ethyl paraben, 120-47-8; hexachlorophene, 11119-93-0, 70-30-4; propyl paraben, 94-13-3; Cosmetics; Pharmaceutic Aids; Preservatives, Pharmaceutical
Chemicals/CAS: chlorocresol, 1321-10-4, 59-50-7; ethyl paraben, 120-47-8; hexachlorophene, 11119-93-0, 70-30-4; propyl paraben, 94-13-3; Cosmetics; Pharmaceutic Aids; Preservatives, Pharmaceutical
Topics
TNO Identifier
230779
ISSN
00219673
Source
Journal of Chromatography, 469, pp. 317-328.
Pages
317-328
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