Serological characterization of a putative human C-type oncornavirus by means of the sepharose bead immunofluorescence assay
article
A putative human C-type oncornavirus isolate was found to be closely related to the simian sarcoma virus by means of the Sepharose bead immunofluorescence assay. Sepharose beads coupled with antisera directed against purified major internal proteins of C-type oncornaviruses were incubated first with tissue culture supernatants and thereafter with antisera conjugated with fluorescein isothiocyanate. The fluorescence of individual beads was then measured microfluorometrically. This technique is a highly sensitive and reproducible assay for the detection of viral proteins. The SKA21-3 culture of rabbit cornea cells, transformed by a murine sarcoma virus pseudotype with a presumably human derived C-type oncornavirus as helper proved to produce abundant amounts of proteins related to the p28 of simian sarcoma virus but not to the p30 of murine leukemia virus. When cells from a tumorous lymph node induced in a rat by the SKA21-3 virus were cocultivated with a canine thymus cell line, A7573, high concentrations of viral proteins were detected in the supernatant after a few passages. The same result was obtained when a leukemic lymph node from a rat inoculated with the presumed human virus, free of the murine sarcoma virus, was cocultivated with the human A204 line.
Chemicals/CAS: Antigens, Viral
Chemicals/CAS: Antigens, Viral
Topics
TNO Identifier
228258
ISSN
02775379
Source
European Journal of Cancer and Clinical Oncology, 13(12), pp. 1397-1403.
Pages
1397-1403
Files
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