Perisinusoidal fat-storing cells are the main vitamin A storage sites in rat liver

article




Hendriks, H.F.J.

Verhoofstad, W.A.M.M.

Brouwer, A.

Leeuw, A.M. de

Knook, D.L.
Highly purified sinusoidal (fat-storing, Kupffer and endothelial cells) and parenchymal cells were isolated to assess the cellular distribution of vitamin A in liver of adult vitamin A-sufficient rats. A modified simple procedure was developed for the purification of fat-storing cells from rat liver. This was achieved by a single centrifugation step in a two-layer density Nycodenz gradient. Endothelial and Kupffer cells were obtained from the same gradient and further purified by centrifugal elutriation. Reverse-phase HPLC analysis showed that fat-storing cells contained about 300-fold the amount of retinyl esters present in parenchymal cells on a mg cell protein basis. In fat-storing cells, the same retinyl esters, viz. retinyl palmitate, retinyl stearate and retinyl oleate, were present as in whole liver. It was also observed that, within 12 h after intravenous injection of chylomicron [3H]retinyl ester, most of the radioactivity had accumulated in the fat-storing cells. It is concluded that fat-storing cells are the main storage sites for vitamin A in rat liver.
Chemicals/CAS: retinol, 68-26-8, 82445-97-4; Chylomicrons; Lipids; retinol palmitate, 79-81-2; retinyl stearate, 631-87-8; Vitamin A, 11103-57-4
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radioisotoperetinolanimal experimentkupffer celllipid storageliverliver endotheliumliver sinusoidnonhumanreticuloendothelial systemAgingAnimalCell SeparationChromatography, High Pressure LiquidChylomicronsFemaleLipidsLiverRatsSupport, Non-U.S. Gov'tTemperatureTissue DistributionVitamin A
TNO Identifier
229942
Repository link
https://resolver.tno.nl/uuid:07b3df7a-6e5b-4383-82b7-9ddd0124a4c9
ISSN
00144827
Source
Experimental Cell Research, 160(1), pp. 138-149.
Pages
138-149
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