Low level exposure to sulfur mustard: Development of a SOP for analysis of albumin adducts and of a system for non-invasive diagnosis on skin
report
Several methods for diagnosis of exposure to chemical warfare agents and other noxious compounds have been transferred to Centers for Disease Control & Prevention.
1. The modified Edman degradation of sulfur mustard-modified hemoglobin
2. The method for diagnosis of exposure to Lewisite
3. The method for diagnosis of exposure to phosgene
In addition, a number of existing methods have been improved:
4. The albumin tripeptide assay for diagnosis of exposure to sulfur mustard
5. The rapid assay for determination of exposure to nerve agents, based on pepsin digestion of human butyrylcholinesterase
In order to circumvent the shortcomings of the current (specific) assays for diagnosis of exposure to OP-anticholinesterases, a generic method for detection of covalently modified human butyrylcholinesterase has been undertaken. Finally, various reference compounds have been synthesized and delivered to CDC.
1. The modified Edman degradation of sulfur mustard-modified hemoglobin
2. The method for diagnosis of exposure to Lewisite
3. The method for diagnosis of exposure to phosgene
In addition, a number of existing methods have been improved:
4. The albumin tripeptide assay for diagnosis of exposure to sulfur mustard
5. The rapid assay for determination of exposure to nerve agents, based on pepsin digestion of human butyrylcholinesterase
In order to circumvent the shortcomings of the current (specific) assays for diagnosis of exposure to OP-anticholinesterases, a generic method for detection of covalently modified human butyrylcholinesterase has been undertaken. Finally, various reference compounds have been synthesized and delivered to CDC.
Topics
TNO Identifier
979356
Publisher
TNO Prins Maurits Laboratorium
Collation
82 p.
Place of publication
Rijswijk