Control system for detection of the illegal use of naturally occuring steroids in calves
article
Within the scope of the National Plan for Hormone Control in The Netherlands, a study was performed to develop a system for control of the illegal use of three naturally occurring hormones [oestradiol-17ß (E2-17ß), testosterone (T), progesterone (P)] for fattening purposes in animal production. Using a specific high-performance liquid chromatographic-radioimmunoassay method, reference values were established for concentrations of E2-17ß, T and P and some of their metabolites in blood plasma and urine from untreated male and female veal calves. E2-17ß levels of both male and female calves were < 0.01 µg/l in blood plasma and < 0.2 µg/l in urine. For male veal calves levels of T and epitestosterone (epiT) in blood plasma and urine varied widely. The P levels were < 0.1-0.3 µg/l in blood plasma and < 0.6-10 µg/l in urine from both male and female calves. To investigate the effect of anabolic treatment on the hormone levels in plasma and excreta, male veal calves were injected, subcutaneously into the dewlap, with a solution containing 20 mg of E2-17ß benzoate and 200 mg of T propionate in 5 ml of arachis oil. Only the levels of E2-17ß and E2-17a in blood plasma and excreta were elevated until about one week after injection, compared with the untreated control calves and the reference values. T and epiT levels were similar in plasma and excreta from both untreated and treated animals.
Topics
TNO Identifier
48586
Source
Journal of Chromatography, 564, pp. 429-444.
Pages
429-444
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