Enumeration of stem cells and progenitor cells in α-thalassemic mice reveals lack of specific regulation of stem cell differentiation
article
Heterozygous α-thalassemic (Hba(th)/+) female mice were investigated for the effect of persistent erythropoietic stress on the number of stem cells and progenitor cells along the erythroid (E), granulocyte-macrophage (GM), and megakaryocyte (Meg) pathways. At the progenitor cell level, compensatory erythropoiesis was demonstrated in the spleen but not in the bone marrow. In the spleen, developmentally early progenitor cells (BFU-E) were expanded two- to threefold and late progenitor cells (CFU-E) five- to sixfold. A comparable expansion of progenitor cells was observed along the GM and Meg pathways. CFU-S numbers were increased in the spleen, but not in the bone marrow. The increases in GM and Meg progenitor cells appeared to result in an inappropriate hemopoiesis: peripheral thrombocyte and monocyte numbers were elevated. However, granulocyte numbers were not significantly increased. It is concluded that the persistently increased erythropoietic demand results in inappropriate production of other hemopoietic cells, most likely because pathway-specific regulatory mechanisms do not influence differentiation at the stem cell level. © 1986 International Society for Experimental Hematology
Topics
alpha thalassemiaanimal cellblood and hemopoietic systemburst forming unit ecell differentiationcolony forming uniterythropoiesiserythropoietic stem cellin vitro studymousenonhumanpriority journalAnimalBlood Cell CountBone MarrowCell DifferentiationErythropoiesisFemaleHematopoietic Stem CellsMaleMiceMice, Mutant StrainsSpleenSupport, Non-U.S. Gov'tThalassemia
TNO Identifier
230075
ISSN
0301472X
Source
Experimental Hematology, 14(4), pp. 303-306.
Pages
303-306
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