Concentration of hemopoietic stem cells using a light-activated cell sorter

article



Engh, G. van den

Visser, J.

Bol, S.

Trask, B.
Light-activated cell sorting although capable of highly specific selection of subpopulations of cells is rate-limited. The method as it now exists is insufficient to supply an adequate number of stem cells for bone marrow transplantation. However, other separation methods using electrophoresis, density gradients, and density centrifugation coupled with cell sorting can improve the yield for clinical utilization.
Chemicals/CAS: Antigens, Surface
Topics




















animal experimentblood and hemopoietic systembone marrowcell separationerythropoietic stem cellmethodologymouseAnimalAntigens, SurfaceCell SeparationCentrifugation, Density GradientCytological TechniquesElectrophoresisHematopoietic Stem CellsLightMiceScattering, RadiationSpectrometry, FluorescenceSupport, Non-U.S. Gov'tSupport, U.S. Gov't, Non-P.H.S.Surface Properties
TNO Identifier
228925
Repository link
https://resolver.tno.nl/uuid:a473dea3-3fb7-4dbf-bddf-dd90fa2cf334
ISSN
03404684
Source
Blood Cells, 6(4), pp. 609-623.
Pages
609-623
Files
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