Protein targeting and secretion in filamentous fungi: a progress report
article
Although the application of filamentous fungi, such as Aspergillus niger for the production of extracellular proteins is well established for several decades, hardly any information is available about the molecular mechanisms of the process of protein secretion in these organisms. Two lines of research initiated towards a systematic analysis of the mechanism of protein targeting and secretion are presented in this paper. 1 - To study routing and targeting of proteins in filamentous fungi the availability of a versatile reporter/carrier protein will be of considerable importance. Experiments towards the identification of such a protein are presented. 2 - In analogy to the situation in Saccharamyces cerevisiae, the availability of defined (conditional) mutations in the secretion pathway will provide very important information about the organisation of the pathway. Therefore, based on results obtained in S. cerevisiae, the cloning of several fungal 'secretion' genes was started. The results of the cloning and characterisation of one of these genes is presented. Chemicals/CAS: Carrier Proteins; DNA, Fungal; Fungal Proteins; Glucuronidase, EC 3.2.1.31
Topics
(conditional) Secretion mutantsAspergillus nigerfilamentous fungireporter/carrier proteinssubcellular compartmentscellular distributionconference paperfungus mutantnonhumanprotein secretionprotein targetingAmino Acid SequenceBase SequenceCarrier ProteinsDNA, FungalEscherichia coliFungal ProteinsFungiGenes, FungalGenes, ReporterGlucuronidaseMicroscopy, ElectronMolecular Sequence Data
TNO Identifier
36703
Source
Antonie van Leeuwenhoek: Journal of Microbiology, 65, pp. 211-216.
Pages
211-216
Files
To receive the publication files, please send an e-mail request to TNO Repository.