Determination of the optical purity of L-selenomethionine-75Se
article
The method developed for the determination of the optical purity of L-selenomethionine-75Se is based on the conversion of the mixture of L-and D-amino acids into the diastereoisomeric derivatives by reaction with (-)-camphorsulphonyl chloride. The diastereoisomeric derivatives are separated by means of thin layer chromatography. From the ratio of the radioactivities of the two spots on the chromatogram, the optical purity is calculated, and given in % D. For a sample of L-methionine-14C the following value was found: 0.53±0.05 (S.D. of mean, n=7). For a sample of DL-methionine-14C the percentage of D found, was: 48.5±0.5 (S. D. of mean, n=7). Of 30 different samples of L-selenomethionine-75Se the percentages of D-isomer were determined in duplicate. From the 30 differences between duplicates the S. D. of a single measurement is calculated to be 0.2. © 1977 Akadémiai Kiadó.
TNO Identifier
228192
ISSN
01340719
Source
Journal of Radioanalytical Chemistry, 35(1), pp. 37-44.
Pages
37-44
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