On the composition and function of the carbohydrate moiety of tissue-type plasminogen activator from human melanoma cells

article


Rijken, D.C.

Emeis, J.J.

Gerwig, G.J.
Two variants (I and II) of tissue-type plasminogen activator (t-PA) from human melanoma cells were separated by Lysine Sepharose chromatography. The carbohydrate compositions of the forms were determined by gas-liquid chromatography. Variant I contained 12.8 g and variant II 7.1 g of carbohydrate per 100 g protein. Both variants contained N-acetylgalactosamine, suggesting O-glycosylation in addition to N-glycosylation. The possible role of N-linked oligosaccharides for the biological activity of t-PA was studied using t-PA secreted by melanoma cells in the presence of tunicamycin, an inhibitior of N-glycosylation. The latter t-PA showed the same plasminogen activating and fibrin binding properties as normally glycosylated t-PA, indicating that N-linked carbohydrate is not involved in the fibrinolytic activity of t-PA. Chemicals/CAS: plasminogen activator, 9039-53-6; Carbohydrates; Fibrin, 9001-31-4; Tissue Plasminogen Activator, EC 3.4.21.68; Tunicamycin, 11089-65-9
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carbohydraten acetylgalactosamineplasminogen activatorblood and hemopoietic systemfibrinolysisgas liquid chromatographyhumanhuman cellmelanomapriority journalCarbohydratesCells, CulturedChromatography, AgaroseFibrinHumanMelanomaSupport, Non-U.S. Gov'tTissue Plasminogen ActivatorTunicamycin
TNO Identifier
229954
Repository link
https://resolver.tno.nl/uuid:abb880a7-aeef-46a1-9003-a5cc73ef379f
ISSN
03406245
Source
Thrombosis and Haemostasis, 54(4), pp. 788-791.
Pages
788-791
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