Hepatic clearance of tissue-type plasminogen activator in rats
article
The clearance of tissue-type plasminogen activator (t-PA) was studied, in rats, by use of a functional assay for t-PA. Half-lives in the circulation were about one minute both for human (melanoma cell-derived) t-PA and for the rat's own t-PA. The clearance of t-PA required an intact liver blood flow. In isolated liver perfusion experiments the hepatic extraction of t-PA did not require any plasma proteins, including fast-acting t-PA inhibitor. Competition experiments, using monosaccharides, suggested that known hepatic glycoprotein receptors were not involved in hepatic t-PA extraction. Chemicals/CAS: heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; plasminogen activator, 9039-53-6; thrombin, 9002-04-4; tissue plasminogen activator, 105913-11-9; tunicamycin, 11089-65-9; Monosaccharides; Tissue Plasminogen Activator, EC 3.4.21.68; Tunicamycin, 11089-65-9
Topics
heparinplasminogen activatorthrombintissue plasminogen activatortunicamycinanimal experimentdose time effect relationdrug clearancehumanintravenous drug administrationliver clearancemelanoma cellnonhumanAnimalLiverMaleMetabolic Clearance RateMonosaccharidesPerfusionRatsSpecies SpecificitySupport, Non-U.S. Gov'tTissue Plasminogen ActivatorTunicamycin
TNO Identifier
229921
ISSN
03406245
Source
Thrombosis and Haemostasis, 54(3), pp. 661-664.
Pages
661-664
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