Large-scale production of extrinsic (tissue-type) plasminogen activator from human melanoma cells
bookPart
This chapter concerns the extrinsic (tissue-type) plasminogen activator (EPA), which differ in many essential aspects from other activators. Large scale production of EPA by melanoma cells cultured on microcarrier beads is described. The scaling-up from monolayer via 3- and 10-liter to 40-liter microcarrier culture did not result in changes in production rate, as a concentration of EPA in the medium of about 15 IU/ml was maintained. This concentration is comparable to reported values. With this technique, production of 4 mg EPA/day is possible with a 40-liter culture. The purified EPA as described in this chapter has been used to study EPA structure and its function in fibrinolysis in vitro and in vivo and to raise poly- and monoclonal antibodies; future studies will focus on EPA's thrombolytic efficacy in animal models and patients.
Topics
TNO Identifier
229444
ISBN
0845132016
Source
Advances in Biotechnological Processes, pp. 97-110.
Publisher
Alan R. Liss Inc
Place of publication
New York, NY, USA
Pages
97-110
Files
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