Inhibition of tissue-type plasminogen activator by conditioned medium from cultured human and porcine vascular endothelial cells

article



Emeis, J.J.

Hinsbergh, V.W.M. van

Verheijen, J.H.

Wijngaards, G.
Conditioned serum-free medium bogh from confluent porcine aortic endothelial cells and from human venous and arterial umbilical cord endothelial cells will inhibit plasminogen activation by tissue-type plasminogen activation. Inhibitory activity in the conditioned medium increases with time, and depends upon protein synthesis. Also, the conditioned medium directly inhibits the amidolytic activity of tissue-type plasminogen activator. Inhibitory activity can be removed from conditioned medium by absorption with immunbilized tissue-type plasminogen activator.
Chemicals/CAS: plasminogen activator, 9039-53-6; Culture Media; Plasminogen Activators, EC 3.4.21.-; Plasminogen Inactivators; Tissue Extracts
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plasminogen activatorblood and hemopoietic systemblood vesselcell cultureendothelium cellgreat blood vesselin vitro studyAnimalAortaBlood VesselsCells, CulturedCulture MediaEndotheliumFemaleHumanPlasminogen ActivatorsPlasminogen InactivatorsSwineTissue ExtractsUmbilical Cord
TNO Identifier
229335
Repository link
https://resolver.tno.nl/uuid:ce64a6ba-e633-44ad-999c-078051c8d56c
ISSN
0006291X
Source
Biochemical and Biophysical Research Communications, 110(2), pp. 392-398.
Pages
392-398
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