Simultaneous determination of the major acidic metabolites of catecholamines and serotonin in urine by liquid chromatography with electrochemical detection after a one-step sample clean-up on Sephadex G-10; influence of vanilla and banana ingestion
article
A simple method is described for the simultaneous determination of vanilmandelic acid (VMA), 3,4-dihydroxyphenylacetic acid (DOPAC), 5-hydroxyindole-3-acetic acid (5-HIAA) and homovanillic acid (HVA) in urine. The compounds are isolated by a one-step sample clean-up on Sephadex G-10, separated by ion-pair reversed-phase liquid chromatography and detected electrochemically. A single analysis is completed within 65 min. Sample clean-up did not cause losses of the compounds of interest. The detection limits in urine were 0.4, 0.8, 1.0 and 1.6 μmol/l for VMA, DOPAC, 5-HIAA and HVA, respectively. 3,4-Dihydroxymandelic acid and vanillic acid (VA) were also detectable, but, under the chromatographic conditions used, they were not resolved from interfering components. VA and 5-HIAA could be analysed separately in the Sephadex G-10 eluate if more restrictive sampling conditions were used. Ingestion of bananas caused an increase of VMA, DOPAC, 5-HIAA and HVA in 24-h urine. After ingestion of vanilla an increased excretion of VA was observed, while the excretion of VMA, DOPAC and HVA was unaffected. Chemicals/CAS: 3,4 dihydroxyphenylacetic acid, 102-32-9; 5 hydroxyindoleacetic acid, 1321-73-9, 54-16-0; homovanillic acid, 306-08-1; vanilmandelic acid, 55-10-7; 3,4-Dihydroxyphenylacetic Acid, 102-32-9; Benzaldehydes; Catecholamines; Homovanillic Acid, 306-08-1; Hydroxyindoleacetic Acid, 54-16-0; Serotonin, 50-67-9; vanillin, 121-33-5; Vanilmandelic Acid, 55-10-7
Topics
TNO Identifier
230610
ISSN
03784347
Source
Journal of Chromatography - Biomedical Applications, 424(2), pp. 273-283.
Pages
273-283
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