Plasminogen activator regulation studies following transfection of human endothelial cells
article
Transfection of human endothelial cells by the calcium phosphate precipitation method has been used to study the gene regulation of plasminogen activator (PA). The method of transfection was optimized, resulting in efficiencies of 1 to 10% and 4 to 20% for human umbilical vein endothelial cells and the hybrid cell line EA. hy926, respectively. Using u-PA-, t-PA- and PAI-1-CAT-promoter constructs a transient CAT-expression was observed. Promoter constructs of different length showed expression of variable amounts of CAT-activity, suggesting a role for enhancer or repressor elements. Stimulation of cells transfected with a 72 bp- or a 2212 bp u-PA-promoter construct with PMA or TNF resulted in induction of only the larger fragment.
TNO Identifier
232528
ISSN
02689499
Source
Fibrinolysis, 8(Suppl. 2), pp. 19-21.
Pages
19-21
Files
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