Biochemisch en electronenmicroscopisch onderzoek van hyperlipoproteinaemia en xanthomatosis ; proefschrift Leiden
article
Biochemical and ultrastructural investigations on hyperlipoproteinaemia and xanthomatosis - The clinical findings differ distinctly in patients with different types of hyperlipoproteinaemia and xanthomatosis. The effects of the lipoprotein classes on dermal and vascular tissue are different. The concentration of apoprotein B (= LOL and VLDL) in interstitial fluid was investigated and proved to be 15% of the apoprotein B serum concentration. The concentration of apoprotein A1 in interstitial fluid proved to be 25 % of the serum concentration. Ultrastructural studies of xanthomatous tissue demonstrated the presence of small particles surrounding the vascular tissue and foamcells in the dermis. These particles had a diameter of 30 nm and resembled LDL particles embedded in a fibrin matrix. Ultrastructural experiments of cultured fibroblasts incubated with LDL enabled us to investigate further the interaction of LDL with cultured cells. The LDL, specifically bound to the plasma membrane, proved to be localized mainly in indented regions with a diameter of 0.15-0.30 μ and a fuzzy coat at the cytoplasmic side. Cultured fibroblasts of a patient homozygous for familial hypercholesterolaemia demonstrated no binding of LDL in these indented regions.
TNO Identifier
287849
Source
Hart bulletin, 11(juni), pp. 69-72.
Pages
69-72
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