The role of apolipoprotein E in the elimination of liposomes from blood by hepatocytes in the mouse

article







Yan, X.

Kuipers, F.

Havekes, L.M.

Havinga, R.

Dontje, B.

Poelstra, K.

Scherphof, G.L.

Kamps, J.A.A.M.
We evaluated the role of apolipoprotein E (apoE) in the clearance of neutral and negatively charged liposomes by hepatocytes in apoE-deficient mice. Negatively charged liposomes were cleared at identical rates in apoE-deficient and wild-type mice; neutral liposomes were cleared at a 3.6-fold slower rate in apoE-deficient mice. ApoE deficiency did not affect hepatic uptake of negatively charged liposomes but lowered that of neutral liposomes >5-fold. Hepatocyte uptake of neutral liposomes was reduced >20-fold in apoE-deficient mice; that of negatively charged liposomes remained unchanged. We conclude that uptake of neutral liposomes by hepatocytes is nearly exclusively apoE-mediated. © 2005 Elsevier Inc. All rights reserved. Chemicals / CAS: Apolipoproteins E; Liposomes
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Biomedical ResearchApolipoprotein E-deficient miceHepatocytesLiposome opsonizationapolipoprotein Eliposomeanimal cellanimal experimentanimal tissuecell transportcontrolled studyevaluationknockout mouseliver cellnonhumanprotein functionprotein transportratspecies comparisonwild typeAnimalsApolipoproteins ECells, CulturedHepatocytesLiposomesLiverMaleMetabolic Clearance RateMiceOrgan SpecificityTissue Distribution
TNO Identifier
238389
Repository link
ISSN
0006291X
Source
Biochemical and Biophysical Research Communications, 328(1), pp. 57-62.
Pages
57-62
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