The effect of 40 kHz ultrasound on tissue plasminogen activator-induced clot lysis in three in vitro models
article
In a previous study, high-frequency ultrasound (US) (3 MHz) was shown to enhance in vitro fibrinolysis through enhanced supply of plasminogen to the clot surface. The application of high-frequency US is limited in vivo, however, due to tissue heating. We continued our research using low-frequency US with less tissue heating and improved penetration of the US. Three different in vitro models, internal plasma clot lysis and external lysis with compacted and noncompacted plasma clots, were used to determine the magnitude of the effect of low-frequency US (40 kHz; 0.5 W/cm2) on tissue plasminogen activator-induced lysis and to elucidate the mechanisms behind the effect. US enhanced lysis in all three models, with the largest effects (fourfold) being in the compacted plasminogen-poor clot model. Plasminogen supply to the clot surface was again shown to be an important contributor to US-enhanced lysis. (E-mail: d.rijken@erasmusmc.nl) © 2004 World Federation for Ultrasound in Medicine & Biology.-- streptokinase, 9002-01-1; tissue plasminogen activator, 105913-11-9; Fibrinolytic Agents; Plasminogen Activators, EC 3.4.21.-; Plasminogen, 9001-91-6; Tissue Plasminogen Activator, EC 3.4.21.68
Topics
Biomedical ResearchThrombolysisTissue-type plasminogen activatorUltrasoundBiological organsBiomaterialsLiving systems studiesUltrasonicsFibrinolysisPlasma clot lysisTissue heatingTissuealteplasestreptokinasetissue plasminogen activatorblood clotblood clot lysisdisease modelheatingCombined Modality TherapyFibrinolysisFibrinolytic AgentsHeatHumansPlasminogenPlasminogen ActivatorsThrombolytic TherapyThrombosisTissue Plasminogen ActivatorUltrasonic Therapy
TNO Identifier
238063
Repository link
ISSN
03015629
Source
Ultrasound in Medicine and Biology, 30(11), pp. 1545-1552.
Pages
1545-1552
Files
To receive the publication files, please send an e-mail request to TNO Repository.