Age-related changes in lymphokine production related to a decreased number of CD45RB(hi) CD4+ T cells
article
The ability of CD4+ T cells from CBA/Rij mice to produce interleukin (IL) 2 after stimulation with anti-CD3, concanavalin A, or the combination of phorbol 12-myristate 13-acetate and ionomycin declines during aging.This phenomenon was accompanied by an increased production of IL 4 and interferon-γ. These age-related changes in lymphokine production correlated with the decrease in the percentage of CD45RB(hi) CD4+ T cells from about 80% in 2-month-old to about 40% in 27-month-old mice.This phenotypic shift was responsible for the decline in IL 2 production, because in young and in old mice CD45RB(hi) CD4+ T cells were more potent IL 2 producers than CD45RB(lo) cells. Moreover, old CD45RB(hi) CD4+ T cells produced less IL 2 than their young counterparts. Proliferative responses by T cells from old mice were lower than those of young mice, regardless whether the cultures were supplemented with IL 2, IL 4 or both lymphokines. As far as CD4+ T cells were concerned, this hyporesponsiveness was found in the CD45RB(lo) as well as in the CD45RB(hi) CD4+ T ccll population. Chemicals/CAS: gamma interferon, 82115-62-6; Antigens, CD45; Antigens, Differentiation; Cytokines; Histocompatibility Antigens; Interferon Type II, 82115-62-6; Interleukin-2; Interleukin-4, 207137-56-2
Topics
TNO Identifier
231472
ISSN
00142980
Source
European Journal of Immunology, 21(2), pp. 273-281.
Pages
273-281
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