Important parameters in liquid chromatography-continuous-flow fast atom bombardment mass spectrometry
article
Continuous-flow fast atom bombardment (CF-FAB) is an attractive technique of interfacing reversed-phase high-performance liquid chromatography and mass spectrometry (LC-MS). Some of the important parameters affecting the performance of CF-FAB were studied. The wettability of the CF-FAB target and liquid film properties on the surface of the CF-FAB target play an important role in obtaining stable performance in LC-CF-FAB-MS. The wettability of the target can be improved by an acid treatment. The thermal conductivity of the CF-FAB target material together with the flow-rate and the mobile phase composition influence the liquid film properties of the target. The optimum supply rate of glycerol is observed to be 0.3 μl/min for LC-CF-FAB-MS under the studied conditions. Some bioanalytical examples of LC-CF-FAB-MS are shown.
TNO Identifier
63730
ISSN
00219673
Source
Journal of Chromatography, 511, pp. 35-47.
Pages
35-47
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