Structural analysis of the α-d-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme
article
The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1→3, 1→6)-α-d-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and 1D/2D <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows:{A figure is presented}. © 2008 Elsevier Ltd. All rights reserved.
Chemicals/CAS: periodate, 15056-35-6; Glucans; Glycosyltransferases, 2.4.-; Periodic Acid, 10450-60-9; Polysaccharides, Bacterial; alternansucrase, 2.4.1.140; metaperiodate, 7790-28-5
Chemicals/CAS: periodate, 15056-35-6; Glucans; Glycosyltransferases, 2.4.-; Periodic Acid, 10450-60-9; Polysaccharides, Bacterial; alternansucrase, 2.4.1.140; metaperiodate, 7790-28-5
Topics
α-d-Glucan<sup>1</sup>H NMR spectroscopyGTF180 GlucansucraseLactobacillus reuteri 180Polysaccharide structural analysisGlucansucraseLactobacillus reuteri 180Linkage analysisPolysaccharide structural analysisEnzymesNuclear magnetic resonance spectroscopyOligosaccharidesOxidationBacteriaLactobacillus reuterilinkage analysismass spectrometrynuclear magnetic resonanceoxidationproton nuclear magnetic resonanceCarbohydrate SequenceGlucansGlycosyltransferasesHydrolysisLactobacillus reuteriMagnetic Resonance SpectroscopyMolecular Sequence DataOxidation-ReductionPeriodic AcidPolysaccharides, BacterialSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationLactobacillus reuteri
TNO Identifier
280045
ISSN
00086215
Source
Carbohydrate Research, 343(7), pp. 1237-1250.
Pages
1237-1250
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