Reliable and sensitive high-performance liquid chromatographic method with fluorometric detection for the analysis of vitamin B-6 in foods and feeds
article
A high-performance liquid chromatographic (HPLC) method with fluorometric detection is described for the analysis of vitamin B-6 in foods and feeds. A simple extraction of the sample with a 5% trichloroacetic acid solution was found to provide complete extraction. The phosphate group was enzymatically removed from the phosphorylated vitamers to facilitate HPLC analysis. The unphosphorylated B-6 vitamers were fully separated by paired-ion reversed-phase (C18) HPLC within 35 min and detected by fluorometry. 4-Deoxypyridoxine was used as internal standard. High recovery and precision were obtained. The range was from 0.02 to 25 μg/g. Varying the composition of the mobile phase changed the capacity factors of the vitamers, an effect which can be used to deal with interferences. The vitamin B-6 content of several foods and feeds was determined with the developed HPLC method and the classical microbiological method using Saccharomyces uvarum. The higher contents measured by HPLC (average difference 40%) could be explained for the greater part by systematic errors in the microbiological method. The results obtained indicate that the described HPLC procedure can serve as a simple and reliable method for the analysis of vitamin B-6 in foods and feeds. © 1994 American Chemical Society.
Topics
TNO Identifier
66281
ISSN
00218561
Source
Journal of Agricultural and Food Chemistry, 42(7), pp. 1475-1480.
Pages
1475-1480
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