Structural identification, neuronal synthesis, and role in male copulation of myomodulin-A of Lymnaea : a study involving direct peptide profiling of nervous tissue by mass spectrometry
article
We used a strategy combining immunodetection, peptide chemistry, and a novel method, direct peptide fingerprinting of neurons and small pieces of nerve by using matrix-assisted laser desorption ionization mass spectrometry, to structurally identify and localize the neuropeptide myomodulin-A in the mollusc, Lymnaea stagnalis. Lymnaea myomodulin appeared to be identical to Aplysia myomodulin-A and is produced by many central neurons, including neurons located in the ventral lobe of the right cerebral ganglion that innervate the penis complex via the penis nerve. Myomodulin-A could also be characterized from the penis complex, and physiological concentrations of the peptide enhanced the relaxation rate of electrically induced contractions of the penis retractor muscle in vitro in a dose-dependent fashion.
Chemicals/CAS: catch-relaxing peptide (Mytilus); myomodulin, 110570-93-9; Neuropeptides; Oligopeptides
Chemicals/CAS: catch-relaxing peptide (Mytilus); myomodulin, 110570-93-9; Neuropeptides; Oligopeptides
Topics
AnimalCopulationImmunoblottingImmunohistochemistryLymnaeaMaleNeuronsNeuropeptidesOligopeptidesSpectrum Analysis, MassStructure-Activity RelationshipSupport, Non-U.S. Gov'tAnimaliaAplysiaGastropodaLymnaeaLymnaea stagnalisMolluscaMyo-modulatory peptideReproductive behaviorDirect mass spectrometry of nervous tissueEdman degradation
TNO Identifier
51887
ISSN
0169328X
Source
Molecular Brain Research, 25(3-4), pp. 355-358.
Pages
355-358
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