Development of an automated on-line pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents

article






Carol-Visser, J.

Schans, M.J. van der

Fidder, A.

Hulst, A.G.

Baar, B.L.M. van

Irth, H.

Noort, D.
Rapid monitoring and retrospective verification are key issues in protection against and non-proliferation of chemical warfare agents (CWA). Such monitoring and verification are adequately accomplished by the analysis of persistent protein adducts of these agents. Liquid chromatography-mass spectrometry (LC-MS) is the tool of choice in the analysis of such protein adducts, but the overall experimental procedure is quite elaborate. Therefore, an automated on-line pepsin digestion-LC-MS configuration has been developed for the rapid determination of CWA protein adducts. The utility of this configuration is demonstrated by the analysis of specific adducts of sarin and sulfur mustard to human butyryl cholinesterase and human serum albumin, respectively. © 2008 Elsevier B.V. All rights reserved.
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Chemical warfare agentsMass spectrometryProtein adductBiological materialsBody fluidsChemical analysisChemical warfareChemicalsChromatographic analysisChromatographyCivil defenseFluidsHigh performance liquid chromatographyIndustrial chemicalsInitiators (chemical)Liquid chromatographyMass spectrometersMass spectrometryMilitary operationsMonitoringSpectrometersSpectrometrySpectrum analysisStabilizers (agents)SulfurButyryl cholinesteraseChemical warfare agents (CWA)Elsevier (CO)Human Serum Albumin (HSA)Liquid chromatography tandem mass spectrometry (LC MS MS)Liquid chromatography/mass spectrometry (LC/MS)Non proliferationRapid analysisRapid monitoringSulfur mustard (SM)High pressure liquid chromatographyChemical warfare agentCholinesterase 9001-08-5Human serum albuminMustard gasPepsin ASarinAnalytic methodAutomationEnzyme degradationLiquid chromatographyProtein analysisTandem mass spectrometryHuman serum albumin 9048-49-1Mustard gas 505-60-2Pepsin A 9001-75-6Sarin 107-44-8Serum Albumin
TNO Identifier
183028
Repository link
https://resolver.tno.nl/uuid:8f6eede6-eb98-4290-ae84-27b164b5e40a
ISSN
1570-0232
Source
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 870(1), pp. 91-97.
Pages
91-97
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