Efficient whole-cell biotransformation of 5-(hydroxymethyl)furfural into FDCA 25-furandicarboxylic acid

article



Koopman, F.

Wierckx, N.

Winde, J.H. de

Ruijssenaars, H.J.
2,5-Furandicarboxylic acid (FDCA) is a promising bio-based platform chemical that may serve as a 'green' substitute for terephthalate in polyesters. Recently, a novel HMF/furfural oxidoreductase from Cupriavidus basilensis HMF14 was identified that converts 5-(hydroxymethyl)furfural (HMF) into FDCA. The hmfH gene encoding this oxidoreductase was introduced into Pseudomonas putida S12 and the resulting wholecell biocatalyst was employed to produce FDCA from HMF. In fed-batch experiments using glycerol as the carbon source, 30.1 g l-1 of FDCA was produced from HMF at a yield of 97%. FDCA was recovered from the culture broth as a 99.4% pure dry powder, at 76% recovery using acid precipitation and subsequent tetrahydrofuran extraction. © 2010 Elsevier Ltd. All rights reserved. Chemicals / CAS glycerol, 56-81-5; oxidoreductase, 9035-73-8, 9035-82-9, 9037-80-3, 9055-15-6; 5 hydroxymethylfurfural, 67-47-0; furfural, 98-01-1; 2,5-furandicarboxylic acid; 5-hydroxymethylfurfural, 67-47-0; Dicarboxylic Acids; Furaldehyde, 98-01-1; Furans
Topics




































































2,5-Furandicarboxylic acidBiotransformationHMF/furfural oxidoreductasePseudomonas putida S12AldehydesCarbonFurfuralGene encodingMethanolPrecipitation (meteorology)2 ,5-furandicarboxylic acidsAcid precipitationBiotransformationCupriavidus basilensisOxidoreductasesPseudomonas putidaWhole cell biocatalystsWhole-cell biotransformationsBacteria2,5 furandicarboxylic acid5 (hydroxymethyl)furfuralcarboxylic acid derivativefuran derivativeglyceroloxidoreductasepolyesterunclassified drug2,5 furandicarboxylic acid2,5-furandicarboxylic acid5 hydroxymethylfurfural5-hydroxymethylfurfuraldicarboxylic aciddrug derivativefurfuralbacteriumbiotransformationcarboxylic acidcatalysisenzyme activityexperimental studyextraction methodgene expressionpolymerprecipitation (chemistry)articlebacterial strainbiocatalystbiotransformationcontrolled studyCupriavidusfed batch culturenonhumanprecipitationpriority journalPseudomonas putidasolvent extractionwhole cellbiocatalysisbiotransformationmetabolismPseudomonas putidaCupriavidus basilensisPseudomonas putidaBiocatalysisBiotransformationDicarboxylic AcidsFuraldehydeFuransPseudomonas putida
TNO Identifier
954221
Repository link
https://resolver.tno.nl/uuid:1587c803-be5f-44a4-adce-f24580f57ffc
ISSN
09608524
Source
Bioresource Technology, 101(16), pp. 6291-6296.
Pages
6291-6296
Files
To receive the publication files, please send an e-mail request to TNO Repository.