Morphological and morphometrical differences between the binding sites of low- and high-density lipoproteins on the plasma membrane of cultured human fibroblasts
article
The binding of LDL and HDL on the plasma membrane of cultured human fibroblasts was visualized with an indirect immunoperoxidase technique followed by electronmicroscopy.
LDL binding sites showed a fuzzy layer on the cytoplasmic surface in most instances, whereas HDL binding sites did not exhibit such a layer. Measurenents of the size of the binding sites revealed a significantly larger mean contour length for LDL binding sites than for HDL binding sites. The mean contour lengths of both LDL and HDL binding sites are signiflcantly larger than the mean contour length of randomly chosen, smooth surfaced plasma membrane invaginations. The mean size of either LDL or HDL binding sites is not influenced by incubation with the lipoproteins at different temperatures.
These findings suggest, that cellular uptake of LDL and HDL occurs at different receptor sites on the plasna membrane.
LDL binding sites showed a fuzzy layer on the cytoplasmic surface in most instances, whereas HDL binding sites did not exhibit such a layer. Measurenents of the size of the binding sites revealed a significantly larger mean contour length for LDL binding sites than for HDL binding sites. The mean contour lengths of both LDL and HDL binding sites are signiflcantly larger than the mean contour length of randomly chosen, smooth surfaced plasma membrane invaginations. The mean size of either LDL or HDL binding sites is not influenced by incubation with the lipoproteins at different temperatures.
These findings suggest, that cellular uptake of LDL and HDL occurs at different receptor sites on the plasna membrane.
TNO Identifier
287368
Source
Cell biology international reports, 6(4), pp. 343-351.
Pages
343-351
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