Characterization of normal and regenerating bone marrow cells with a panel of monoclonal antibodies
article
To obtain baseline data for the diagnosis of residua] leukaemia or lymphoma in bone marrow (BM), BM samples of 12 normal adults, 6 patients treated with chemotherapy for solid tumours and 6 patients treated with chemotherapy for leukaemia or lymphoma during remission were assessed with a panel of monoclonal antibodies by fluorescence microscopy immediately following chemotherapy. A large variability between normal individuals was found concerning the percentages of T lymphocytes and T cell subsets, B lymphocytes, HLA‐DR* and OKT104 cells, due to variation in BM composition and, presumably, peripheral blood contamination. The regenerating BM cells also showed a large variability and, in addition, sometimes very high percentages (up to 19%) of CALLA +, cu'sjr, cu + KV and TdT+ cells were found. The interpretation of data on individual BM samples of patients assessed for leukaemia or lymphoma should therefore be related to: (1) the amount of contaminating peripheral blood cells; (2) the cellular composition of the BM samples as determined by May‐Grunwald Giemsa staining; (3) baseline data of age‐matched normal controls and of regenerating BM of patients without haematological diseases.
TNO Identifier
355832
Source
Scandinavian Journal of Haematology, 36(1), pp. 71-78.
Pages
71-78
Files
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