Separation of megakaryocytes from mouse bone marrow by velocity sedimentation

article
A technique for separating mouse bone marrow megakaryocytes by unit gravity velocity sedimentation is described using a modified sedimentation chamber. The velocity sedimentation profile for megakaryocytes comprises three peaks corresponding to sedimentation velocities of 30, 60, and 100 mm/hr. Morphologic examination of megakaryocytes in the 30 mm/hr peak reveals them to be primarily immature with the latter two peaks corresponding to larger, more mature megakaryocytes.
Topics
TNO Identifier
353538
Source
Blood, 43(4), pp. 591-595.
Pages
591-595
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