Use of reagent-inpregnated ('Patho-Tec') test papers in the identification of enterobacteriaceae and similar bacteria

article


Narayan, K.G.

Guinée, P.A.M.

Mossel, D.A.A.
A comparative examination of Patho-Tec reagent-impregnated paper strips and the corresponding classical biochemical tests for the identification of Enterobacteriaceae was made. Patho-Tec papers appeared to be reliable for the cytochrome-oxidase and phenylalanine-deaminase tests. The urease test papers gave false negative reactions in Citrobacter and Enterobacter isolates, but never in Proteus strains. The lysine-decarboxylase test papers gave too many false positive results. These shortcomings of the strips interfere with fast and reliable identification of Salmonella-like Enterobacteriaceae.
Chemicals/CAS: carboxylyase, 9027-22-9; cytochrome c oxidase, 72841-18-0, 9001-16-5; hydrolase, 9027-41-2; urease, 9002-13-5; Aminohydrolases, EC 3.5.4.; Carboxy-Lyases, EC 4.1.1.; Cytochrome-c Oxidase, EC 1.9.3.1; Urease, EC 3.5.1.5
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Carboxy Lyasescarboxylyasecytochrome c oxidasehydrolaseureasearticlecomparative studyEnterobacteriaceaeenzymologyisolation and purificationmicrobiological examinationpaperProteusSalmonellaAminohydrolasesBacteriological TechniquesCarboxy-LyasesComparative StudyCytochrome-c OxidaseEnterobacteriaceaePaperProteusSalmonellaUrease
TNO Identifier
286988
Repository link
https://resolver.tno.nl/uuid:e4281611-a6fe-48b8-8278-a9a7c856b716
Source
Antonie van Leeuwenhoek, 33(1), pp. 184-188.
Pages
184-188
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