Plasma metabolome analysis identifies distinct human metabotypes in the postprandial state with different susceptibility to weight loss-mediated metabolic improvements
article
Health has been defined as the capability of the organism to adapt to challenges. In this study,we tested to what extent comprehensively phenotyped individuals reveal differences in metabolic responses to a standardized mixed meal tolerance test (MMTT) and how these responses change when individuals experience moderate weight loss. Metabolome analysis was used in 70 healthy individuals with profiling of~300 plasmametabolites during an MMTT over 8 h. Multivariate analysis of plasma markers of fatty acid catabolism identified 2 distinct metabotype clusters (AandB). Individuals from metabotypeB showed slower glucose clearance, had increased intra-abdominal adipose tissue mass and higher hepatic lipid levels when compared with individuals from metabotypeA. An NMRbased urine analysis revealed that these individuals also to have a less healthy dietary pattern.After aweight loss of ~5.6 kg over 12wk, only the subjects from metabotype B showed positive changes in the glycemic response during the MMTT and in markers of metabolic diseases. Our study in healthy individuals demonstrates that more comprehensive phenotyping can reveal discrete metabotypes with different outcomes in a dietary intervention and that markers of lipid catabolism in plasma could allow early detection of the metabolic syndrome. FASEB. © 2018 FASEB. All rights reserved.
TNO Identifier
842714
ISSN
08926638
Source
FASEB Journal, 32(10), pp. 5447-5458.
Pages
5447-5458
Files
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